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KMID : 0613820130230020306
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Journal of Life Science
2013 Volume.23 No. 2 p.306 ~ p.310
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Application of Competitive ELISA Method for Estimation of Urinary Aflatoxin M1 Level
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Kim Yong-Dae
Kim Heon
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Abstract
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We compared the efficacy of the competitive ELISA method for measuring the level of urinary aflatoxin M1 (AFM1) with that of the HPLC-fluorescence detector (HPLC-FLD) method. The recovery rate of AFM1 with the ELISA method was 105% (73-124%), and the coefficient of variation of the analysis was 6.85%. The ELISA method showed a 0.20 pg/ml and 0.62 pg/ml limit of detection and limit of quantitation, respectively. In correlation analysis, the two methods showed a very strong and statistically significant correlation (R=0.96, p<0.01). However, in spite of the strong correlation, the ELISA method tended to overestimate the urinary AFM1 concentration compared to the HPLC-FLD method. These results suggest that the competitive ELISA method may be a useful technique for measuring the AFM1 level in high-throughput urine samples, but it needs to be corrected with a regression equation from regression analysis with the HPLC-FLD method.
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KEYWORD
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Urinary aflatoxin M1, competitive ELISA, HPLC-fluorescence detector (HPLC-FLD)
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